Journal: Molecular Medicine Reports
Article Title: Protective effects of the knockdown of lncRNA AK139328 against oxygen glucose deprivation/reoxygenation-induced injury in PC12 cells
doi: 10.3892/mmr.2021.12260
Figure Lengend Snippet: Effects of AK139328 silencing on cell apoptosis in PC12 cells exposed to OGD/R. (A) Flow cytometry was performed to determine the levels of cell apoptosis in OGD/R-induced PC12 cells transfected with shRNA-AK139328 or shRNA. (B) Protein expression levels of Bcl-2, Bax, cleaved caspase-3 and caspase-3 in PC12 cells were analyzed using western blotting. Data are expressed as the mean ± SD. *P<0.05, ***P<0.001 vs. control; ### P<0.001 vs. OGD/R + shRNA. OGD/R, oxygen glucose deprivation/reoxygenation; shRNA, short hairpin RNA.
Article Snippet: The data were analyzed using flow cytometry software (iSortTM Automated Cell Sorter; version A.0; Thermo Fisher Scientific, Inc.).
Techniques: Flow Cytometry, Transfection, shRNA, Expressing, Western Blot, Control